Expression of the proprotein convertases PC1 and PC2 mRNAs in thyrotropin releasing hormone neurons of the rat paraventricular nucleus of hypothalamus
Identifieur interne : 003B86 ( Main/Exploration ); précédent : 003B85; suivant : 003B87Expression of the proprotein convertases PC1 and PC2 mRNAs in thyrotropin releasing hormone neurons of the rat paraventricular nucleus of hypothalamus
Auteurs : Edith Sánchez ; Jean-Louis Charli [Mexique] ; Claudia Morales ; Gabriel Corkidi [Mexique] ; Nabil G. Seidah [Canada] ; Patricia Joseph-Bravo ; Rosa Maria UribeSource :
- Brain Research [ 0006-8993 ] ; 1997.
English descriptors
- KwdEn :
Abstract
PC1 and PC2 are subtilisin-like processing enzymes capable of cleaving thyrotropin releasing hormone (TRH) precursor (pro-TRH) at paired basic residues in vitro. In the paraventricular nucleus of the hypothalamus (PVN), pro-TRH is synthesized to control adenohypophysial thyrotropin and prolactin release. Biochemical and immunological approaches have shown that in the hypothalamus, pro-TRH is extensively cleaved at pairs of basic amino acids. We quantified, by two different approaches, in situ hybridization (ISH) on consecutive cryostat sections or double label ISH, the proportion of PVN TRH neurons containing either PC1 or PC2 mRNAs. Both techniques gave similar results: PC2 mRNA was present in 60–70% of TRH neurons, and PC1 mRNA in 37–46%. Values were similar in the anterior and medial parts of the parvocellular PVN. TRH neurons containing either PC1 or PC2 mRNA were found throughout the areas containing TRH cells without any evidence of anatomical segregation. These results suggest a biochemical heterogeneity in PVN TRH biosynthetic machinery.
Url:
DOI: 10.1016/S0006-8993(97)00280-1
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002910
- to stream Istex, to step Curation: 002910
- to stream Istex, to step Checkpoint: 001680
- to stream Main, to step Merge: 004238
- to stream Main, to step Curation: 003B86
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Expression of the proprotein convertases PC1 and PC2 mRNAs in thyrotropin releasing hormone neurons of the rat paraventricular nucleus of hypothalamus</title>
<author><name sortKey="Sanchez, Edith" sort="Sanchez, Edith" uniqKey="Sanchez E" first="Edith" last="Sánchez">Edith Sánchez</name>
</author>
<author><name sortKey="Charli, Jean Louis" sort="Charli, Jean Louis" uniqKey="Charli J" first="Jean-Louis" last="Charli">Jean-Louis Charli</name>
</author>
<author><name sortKey="Morales, Claudia" sort="Morales, Claudia" uniqKey="Morales C" first="Claudia" last="Morales">Claudia Morales</name>
</author>
<author><name sortKey="Corkidi, Gabriel" sort="Corkidi, Gabriel" uniqKey="Corkidi G" first="Gabriel" last="Corkidi">Gabriel Corkidi</name>
</author>
<author><name sortKey="Seidah, Nabil G" sort="Seidah, Nabil G" uniqKey="Seidah N" first="Nabil G" last="Seidah">Nabil G. Seidah</name>
</author>
<author><name sortKey="Joseph Bravo, Patricia" sort="Joseph Bravo, Patricia" uniqKey="Joseph Bravo P" first="Patricia" last="Joseph-Bravo">Patricia Joseph-Bravo</name>
</author>
<author><name sortKey="Uribe, Rosa Maria" sort="Uribe, Rosa Maria" uniqKey="Uribe R" first="Rosa Maria" last="Uribe">Rosa Maria Uribe</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:90C47C6DF91234AAE93F8823424A28BB0A2C3B8E</idno>
<date when="1997" year="1997">1997</date>
<idno type="doi">10.1016/S0006-8993(97)00280-1</idno>
<idno type="url">https://api-v5.istex.fr/document/90C47C6DF91234AAE93F8823424A28BB0A2C3B8E/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002910</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002910</idno>
<idno type="wicri:Area/Istex/Curation">002910</idno>
<idno type="wicri:Area/Istex/Checkpoint">001680</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001680</idno>
<idno type="wicri:doubleKey">0006-8993:1997:Sanchez E:expression:of:the</idno>
<idno type="wicri:Area/Main/Merge">004238</idno>
<idno type="wicri:Area/Main/Curation">003B86</idno>
<idno type="wicri:Area/Main/Exploration">003B86</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Expression of the proprotein convertases PC1 and PC2 mRNAs in thyrotropin releasing hormone neurons of the rat paraventricular nucleus of hypothalamus</title>
<author><name sortKey="Sanchez, Edith" sort="Sanchez, Edith" uniqKey="Sanchez E" first="Edith" last="Sánchez">Edith Sánchez</name>
<affiliation><wicri:noCountry code="subField">62271</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Charli, Jean Louis" sort="Charli, Jean Louis" uniqKey="Charli J" first="Jean-Louis" last="Charli">Jean-Louis Charli</name>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Mexique</country>
</affiliation>
</author>
<author><name sortKey="Morales, Claudia" sort="Morales, Claudia" uniqKey="Morales C" first="Claudia" last="Morales">Claudia Morales</name>
<affiliation><wicri:noCountry code="subField">62271</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Corkidi, Gabriel" sort="Corkidi, Gabriel" uniqKey="Corkidi G" first="Gabriel" last="Corkidi">Gabriel Corkidi</name>
<affiliation wicri:level="1"><country xml:lang="fr">Mexique</country>
<wicri:regionArea>Centro de Instrumentos, Universidad Nacional Autónoma de México, Mexico D.F.</wicri:regionArea>
<wicri:noRegion>Mexico D.F.</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Seidah, Nabil G" sort="Seidah, Nabil G" uniqKey="Seidah N" first="Nabil G" last="Seidah">Nabil G. Seidah</name>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>J.A. DeSeve Laboratories of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Montreal, Que.</wicri:regionArea>
<wicri:noRegion>Que.</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Joseph Bravo, Patricia" sort="Joseph Bravo, Patricia" uniqKey="Joseph Bravo P" first="Patricia" last="Joseph-Bravo">Patricia Joseph-Bravo</name>
<affiliation><wicri:noCountry code="subField">62271</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Uribe, Rosa Maria" sort="Uribe, Rosa Maria" uniqKey="Uribe R" first="Rosa Maria" last="Uribe">Rosa Maria Uribe</name>
<affiliation><wicri:noCountry code="subField">62271</wicri:noCountry>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Brain Research</title>
<title level="j" type="abbrev">BRES</title>
<idno type="ISSN">0006-8993</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1997">1997</date>
<biblScope unit="volume">761</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="77">77</biblScope>
<biblScope unit="page" to="86">86</biblScope>
</imprint>
<idno type="ISSN">0006-8993</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0006-8993</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosynthesis</term>
<term>Convertase</term>
<term>Hybridization, in situ</term>
<term>Hypothalamus</term>
<term>PC1</term>
<term>PC2</term>
<term>Paraventricular nucleus</term>
<term>Processing</term>
<term>Thyrotropin releasing hormone (TRH)</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">PC1 and PC2 are subtilisin-like processing enzymes capable of cleaving thyrotropin releasing hormone (TRH) precursor (pro-TRH) at paired basic residues in vitro. In the paraventricular nucleus of the hypothalamus (PVN), pro-TRH is synthesized to control adenohypophysial thyrotropin and prolactin release. Biochemical and immunological approaches have shown that in the hypothalamus, pro-TRH is extensively cleaved at pairs of basic amino acids. We quantified, by two different approaches, in situ hybridization (ISH) on consecutive cryostat sections or double label ISH, the proportion of PVN TRH neurons containing either PC1 or PC2 mRNAs. Both techniques gave similar results: PC2 mRNA was present in 60–70% of TRH neurons, and PC1 mRNA in 37–46%. Values were similar in the anterior and medial parts of the parvocellular PVN. TRH neurons containing either PC1 or PC2 mRNA were found throughout the areas containing TRH cells without any evidence of anatomical segregation. These results suggest a biochemical heterogeneity in PVN TRH biosynthetic machinery.</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
<li>Mexique</li>
</country>
</list>
<tree><noCountry><name sortKey="Joseph Bravo, Patricia" sort="Joseph Bravo, Patricia" uniqKey="Joseph Bravo P" first="Patricia" last="Joseph-Bravo">Patricia Joseph-Bravo</name>
<name sortKey="Morales, Claudia" sort="Morales, Claudia" uniqKey="Morales C" first="Claudia" last="Morales">Claudia Morales</name>
<name sortKey="Sanchez, Edith" sort="Sanchez, Edith" uniqKey="Sanchez E" first="Edith" last="Sánchez">Edith Sánchez</name>
<name sortKey="Uribe, Rosa Maria" sort="Uribe, Rosa Maria" uniqKey="Uribe R" first="Rosa Maria" last="Uribe">Rosa Maria Uribe</name>
</noCountry>
<country name="Mexique"><noRegion><name sortKey="Charli, Jean Louis" sort="Charli, Jean Louis" uniqKey="Charli J" first="Jean-Louis" last="Charli">Jean-Louis Charli</name>
</noRegion>
<name sortKey="Corkidi, Gabriel" sort="Corkidi, Gabriel" uniqKey="Corkidi G" first="Gabriel" last="Corkidi">Gabriel Corkidi</name>
</country>
<country name="Canada"><noRegion><name sortKey="Seidah, Nabil G" sort="Seidah, Nabil G" uniqKey="Seidah N" first="Nabil G" last="Seidah">Nabil G. Seidah</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003B86 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003B86 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Canada |area= ParkinsonCanadaV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:90C47C6DF91234AAE93F8823424A28BB0A2C3B8E |texte= Expression of the proprotein convertases PC1 and PC2 mRNAs in thyrotropin releasing hormone neurons of the rat paraventricular nucleus of hypothalamus }}
This area was generated with Dilib version V0.6.29. |